Research Paper Volume 10, Issue 12 pp 3897—3909

Melatonin prevents deterioration in quality by preserving epigenetic modifications of porcine oocytes after prolonged culture

Figure 2. The effect of melatonin on histone methylation at H3K4me2 of prolonged-culture oocytes. Porcine oocytes matured in vitro were continuously cultured in medium supplemented with or without 10−3 M melatonin for 24 h. (A) Representative images of fresh, prolonged-culture and prolonged-culture + Mel oocytes stained with H3K4me2 antibody. (B) Quantitative analysis of H3K4me2 fluorescence intensity. The data are presented as mean ± SEM of at least three independent experiments. PC, prolonged-culture; Mel, melatonin; Scale bar=100 μm. Data are expressed as the mean ± SEM, ****P<0.0001.