Research Paper Volume 10, Issue 12 pp 3897—3909

Melatonin prevents deterioration in quality by preserving epigenetic modifications of porcine oocytes after prolonged culture

Figure 3. Effect of melatonin on histone methylation at H3K27me2 of prolonged-culture oocytes. Porcine oocytes matured in vitro were continuously cultured in medium supplemented with or without 10−3 M melatonin for 24 h. (A) Representative images of fresh, prolonged-culture and prolonged-culture + Mel oocytes stained with H3K27me2 antibody. (B) Quantitative analysis of H3K27me2 fluorescence intensity. PC, prolonged-culture; Mel, melatonin, Scale bar=100 μm. The data are expressed as the mean ± SEM of at least three independent experiments, **** P<0.0001.