Research Paper Volume 10, Issue 12 pp 3897—3909

Melatonin prevents deterioration in quality by preserving epigenetic modifications of porcine oocytes after prolonged culture

Figure 4. Effects of melatonin on global genomic DNA methylation and DNMT expression in prolonged-culture oocytes. Porcine oocytes matured in vitro were continuously cultured in medium supplemented with or without 10−3 M melatonin for 24 h. (A) Representative images of fresh, prolonged-culture, and prolonged-culture + Mel oocytes stained with 5mc antibody. (B) Quantitative analysis of 5mc fluorescence intensity. (C) Expression of DNMT1, DNMT3A, and DNMT3B in fresh, PC, and PC + Mel oocytes. The data are presented as the mean ± SEM of at least three independent experiments. Scale bar=100 μm; PC, prolonged-culture; Mel, melatonin; **P<0.01, *** P<0.001, **** P<0.0001.