SIN-3 as a key determinant of lifespan and its sex dependent differential role on healthspan in Caenorhabditis elegans

Figure 2.Loss of sin-3 causes reproductive defects in C. elegans. Graphical representation of (a) total number of eggs laid in the entire reproductive span of worms, (b) total number of eggs that were viable and (c) percentage of males in the viable progeny after self-fertilization and mating. (d) Loss of sin-3 causes AVID phenotype defect in C. elegans measured as percentage of worms showing ruptured vulva and protrusion of intestine and uterus. (e-g) Graphical representation for the quantification of red, green and the ratio of red/green for JC-1 staining respectively depicting the hypopolarisation of mitochondrial membrane potential in case in sin-3 deletion in C. elegans (For figure a, b and e-f: ns, non-significant; *p < 0.05; **p < 0.001, ****p < 0.0001 and denotes the comparison with respect to wild-type N2; Two way ANOVA performed # p <0.001 denotes the comparison between sin-3;him-5 and him-5(e1490) Student’s t test performed. For figure (c) and (d) *p < 0.05; **p < 0.001, ****p < 0.0001 two way ANOVA performed).