Figure 5. Loss of sin-3 causes decline in percentage survival of worms after thermal stress insult. Percentage survival with and without acclimatization at 25 °C of (a) N2 hermaphrodite (b) him-5(e1490) hermaphrodites and (c) him-5(e1490) males. (d) Loss of sin-3 causes decline in percentage survival after thermal stress insult in both hermaphrodites and (e) males (ns, non-significant; * p < 0.05; Student’s t test was performed). (f) Loss of sin-3 causes decline in the survival after osmotic stress insults in the strains indicated when tested on NGM plates with 500mM NaCl. (g)sin-3 deletion causes significant protein carbonylation. Hermaphrodite and males of the indicated strains were subjected to quantification of protein carbonyl content (ns, non-significant; *p < 0.05; **p < 0.001, ****p < 0.0001 and denotes the comparison with respect to wild-type N2; Two way ANOVA performed # p <0.001 denotes the comparison between sin-3;him-5 and him-5(e1490) Student’s t test performed).