Figure 1. Islets injury induced by STZ or cytokines in vitro. Islets isolated from ICR mice were treated with STZ or TNFα, IL-1β and IFNγ cytokines cocktail. (A) AO/PI staining of islets with STZ, cytokines and control. (B-E) qRT-PCR measured the expression of apoptosis and endoplasmic reticulum stress related genes (Fas, Chop, Bax, Caspase-3) in islets after different treatment. (F, G) FAS and CHOP quantification by western blot. The data are presented as mean±SEM of three independent experiments. *, **, *** denote a significant difference between STZ or cytokines treatment and control (* P<0.05, ** P<0.01, *** P<0.001).