Research Paper Volume 10, Issue 12 pp 3986—3999

Injury factors alter miRNAs profiles of exosomes derived from islets and circulation

Figure 1. Islets injury induced by STZ or cytokines in vitro. Islets isolated from ICR mice were treated with STZ or TNFα, IL-1β and IFNγ cytokines cocktail. (A) AO/PI staining of islets with STZ, cytokines and control. (B-E) qRT-PCR measured the expression of apoptosis and endoplasmic reticulum stress related genes (Fas, Chop, Bax, Caspase-3) in islets after different treatment. (F, G) FAS and CHOP quantification by western blot. The data are presented as mean±SEM of three independent experiments. *, **, *** denote a significant difference between STZ or cytokines treatment and control (* P<0.05, ** P<0.01, *** P<0.001).