Figure 2. Characterization of islets-released exosomes. Exosomes derived from islets culture medium were characterized by NTA, TEM and western blot. (A-C) Particle and size distribution of analyzed derived from control, STZ and cytokines treated mouse islets were mostly within the normal range for exosome size (30-150 nm in diameter). (D-F) TEM of negatively staining showed the morphology of control, STZ and cytokines treated islet exosomes respectively. (G) Western blot analysis of CD63 and CD81 expression in exosomes.