Figure 2. Midlife aging in WT mice was associated with hippocampal gene expression changes largely irrespective of environmental condition. (A) Schematic diagram showing number of DEGs derived from comparing 6- and 12-month-old WT mice. (B) Heatmap of hierarchically clustered z-scores for 1940 DEGs derived from comparing 6- and 12-month-old WT mice across environmental conditions. (C) Scatter plot of gene expression changes between 6- and 12-month-old WT mice with respect to standard (x-axis) and enriched (y-axis) environment. 1251 DEGs within the 50% window around the ordinary diagonal coloured in green. (D) Enriched pathways for 1251 DEGs from (C). Ten most significant terms, their adjusted p values, and overlapping DEG count shown. (E) Venn diagrams comparing DEGs identified between 6- and 12-month-old WT mice with known age-regulated genes in the hippocampus of 24- and 29-month-old mice . (F) Expression changes of C4b, Pisd-ps1, and Sox11 between 6- and 12-month-old WT mice plotted as individual data points with mean ± SEM.