Research Paper Volume 10, Issue 12 pp 4024—4041

SNCA overexpression disturbs hippocampal gene expression trajectories in midlife

Figure 2. Midlife aging in WT mice was associated with hippocampal gene expression changes largely irrespective of environmental condition. (A) Schematic diagram showing number of DEGs derived from comparing 6- and 12-month-old WT mice. (B) Heatmap of hierarchically clustered z-scores for 1940 DEGs derived from comparing 6- and 12-month-old WT mice across environmental conditions. (C) Scatter plot of gene expression changes between 6- and 12-month-old WT mice with respect to standard (x-axis) and enriched (y-axis) environment. 1251 DEGs within the 50% window around the ordinary diagonal coloured in green. (D) Enriched pathways for 1251 DEGs from (C). Ten most significant terms, their adjusted p values, and overlapping DEG count shown. (E) Venn diagrams comparing DEGs identified between 6- and 12-month-old WT mice with known age-regulated genes in the hippocampus of 24- and 29-month-old mice [16]. (F) Expression changes of C4b, Pisd-ps1, and Sox11 between 6- and 12-month-old WT mice plotted as individual data points with mean ± SEM.