Research Paper Volume 10, Issue 12 pp 4152—4165

Mechanistic insight into hyaluronic acid and platelet-rich plasma-mediated anti-inflammatory and anti-apoptotic activities in osteoarthritic mice

Figure 6. In vivo anti-inflammatory and anti-hypertrophic efficacy of HA+PRP in ACLT-OA knee joint. (A) Immunohistochemical (IHC) staining for matrix metalloproteinase (MMP-1) distributions (indicated by black arrows) and their relative quantification in ACLT, HA, PRP and HA+PRP treated groups (B) Alizarin Red S staining and their relative quantification for detection of calcium depositions (red color), indicating chondrocyte hypertrophy in ACLT, HA, PRP and HA+PRP treated groups. *p<0.05, The results are presented as mean ± S.D. for 9 independent experimental replicates.