Research Paper Volume 10, Issue 12 pp 4166—4174

Exercise training augments Sirt1-signaling and attenuates cardiac inflammation in D-galactose induced-aging rats

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Figure 2. Effect of exercise training in TNF alpha associated Inflammatory pathway. (A) Representative Western blots showing protein products of TNF-R, TNF-α, iNOS, p-NFκB and COX-2 extracted from left ventricles of excised hearts (B) The α-tubulin was used as an internal control. (C) Densitometry bars showing the relative protein levels represented by mean values ± SEM. *P< 0.05, **P< 0.01, ***P< 0.001significant differences with respect to control group. #P<0.05, ##P<0.01, ###P< 0.001 significant differences with respect to aging group.