Research Paper Volume 10, Issue 12 pp 4175—4187

Melatonin alleviates meiotic defects in fetal mouse oocytes induced by Di (2-ethylhexyl) phthalate in vitro

Figure 1. Effects of MLT on meiotic progression and DSBs in DEHP-exposed fetal ovaries. (A) Morphology of 12.5 dpc ovaries cultured for 6 days in control, DEHP and DEHP+MLT group in vitro. (B) Western blot analyses of the expression of Sycp3 and γH2afx protein in control, DEHP, and DEHP+MLT groups. (C) Relative expression level of genes Sycp3 and Trp53 in control, DEHP and DEHP+MLT groups. The results were presented as mean ± SEM. *P < 0.05, ** P < 0.01.