Research Paper Volume 10, Issue 12 pp 4197—4212

Hyperoside attenuates renal aging and injury induced by D-galactose via inhibiting AMPK-ULK1 signaling-mediated autophagy

Figure 1. The effects of hyperoside and vitamin E on renal aging and injury induced by D-galactose in vivo. (A) A WB analysis of klotho and p53 in the kidneys of the rats in the control, the 4 week-D-gal and the 8 week-D-gal groups. (B) A WB analysis of klotho and p53 in the kidneys of the rats in the control, the 8 week-D-gal, the D-gal + Hyperoside and the D-gal + VE groups. (C) Immunohistochemical staining of klotho and the percentage of positively stained areas for klotho in the control, the 8 week-D-gal and the D-gal + Hyperoside groups. Scale bar = 20 μm. (D, E) The serum levels of BUN and Scr in the control, the 8 week-D-gal, the D-gal + Hyperoside and the D-gal + VE groups. The data are expressed as the mean ± SD, (n=3), *P < 0.05, **P < 0.01. Abbreviation: WB, Western blot; D-gal, D-galactose; VE, vitamin E; BUN, blood urea nitrogen; Scr, serum creatinine.