Research Paper Volume 10, Issue 12 pp 4213—4223

Caloric restriction is associated with preservation of muscle strength in experimental cancer cachexia

Figure 1. Daily body weight throughout the experiment. Grouped histograms depicting the mean daily bodyweights per group in C26 tumor-bearing (TB) male CD2F1 mice with ad libitum access to chow (dietary reference intake [DRI], n = 10); C26 TB mice on a 30% caloric restriction (CR, n = 10) diet; non-tumor bearing (NTB) mice with matched intake (MI, n = 10); NTB mice on a 30% caloric restriction (n = 10). The vertical dashed lines indicate the timepoint in the experiment in which tumor inoculation was performed in tumor-bearing groups. The vertical bars indicate daily measurements of body weight, ranging from day 0 to 35, for each specified group. Bodyweight was normalized to each animal’s body weight on day 0 and is expressed as the percental difference. Following initiation of 30% CR a rapid decline in body weight was observed prior to tumor inoculation, -10.5% for C26 TB 30% CR mice and -10.6% for NTB 30% CR mice (p < 0.001 for both groups compared to C26 TB DRI). Following tumor inoculation, C26 TB DRI mice experienced a 10.6% drop in bodyweight preceding sacrifice (p = 0.01, paired-sample t-test), whereas C26 TB 30% CR mice had a steady bodyweight in this phase of the experiment. NTB MI mice experienced a 6.4% drop in body weight (p = 0.002, paired-sample t-test) and NTB 30% CR mice experienced a 7.6% drop in body weight (p = 0.004, paired-sample t-test) preceding sacrifice.