Research Paper Volume 11, Issue 7 pp 1990—2002

Hepatic lipid droplet breakdown through lipolysis during hibernation in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Figure 4. Activated cAMP/PKA signal increased ATGL accumulation on the surface of LDs. (A) Nile red staining of isolated LDs; (B) Colloidal blue staining of LDs, total membrane (TM), cytosol (Cyto) and post-nuclear supernatant (PNS) in the liver; (C) Western blot analysis of ATGL protein expression in the liver, LDs and cytosol; (D) Statistics of ATGL protein expression; (E) The cAMP level in the liver; (F) Differential phosphorylation of PKA in the liver.