Research Paper Volume 11, Issue 7 pp 1990—2002

Hepatic lipid droplet breakdown through lipolysis during hibernation in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Figure 5. Phosphorylation of perilipin-5 on LD induced CGI-58-mediated regulation of ATGL activity. (A) Western blotting analysis of perilipin 5 protein expression and perilipin 5 phosphorylation; (B) Statistics of perilipin 5 protein expression in the liver, LDs and cytosol; (C) Statistics of perilipin 5 phosphorylation in LDs; (D) Analysis of the CGI-58/ATGL-perilipin 5 interaction; (E) Statistics of precipitated proteins.