Research Paper Volume 11, Issue 7 pp 2003—2019

Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease

Figure 2. Genistein prevents P31-43 induced epithelial stress response. (A-B) Caco-2 cells were left untreated or incubated with P57-68 or with P31-43 in the presence or absence of VX-770 or genistein. Immunoblot of phospho-ERK 1/2 (A) or PPARγ (B) and densitometric analysis of protein levels relative to β-actin (right) (n=3 independent experiments). Means±SD of triplicates of independent experiments; **p<0.05 or ***p<0.001 untreated or P57-68 versus P31-43, °°p<0.01 P31-43 versus genistein+P31-43, ##p<0.01 P31-43 versus VX-770+P31-43 (ANOVA, Bonferroni post-hoc test). (C) In situ detection of TG2 activity, in Caco-2 cells pulsed with Ca2+, by immunoblotting of the TGM2-catalyzed incorporation of 5-biotinamidopentylamine (BAP) and blotting with anti-biotin antibody (n=3 independent experiments). Data information: The blots are representative of one experiment for group of treatment.