Research Paper Volume 11, Issue 8 pp 2202—2216

Doxycycline, Azithromycin and Vitamin C (DAV): A potent combination therapy for targeting mitochondria and eradicating cancer stem cells (CSCs)

Figure 7. Combining low-dose Azithromycin and Doxycycline with Vitamin C (DAV), dramatically inhibits metabolism: Seahorse profiles. The metabolic profile of MCF7 cell monolayers pre-treated with the triple combination (1 μM Doxycycline, 1 μM Azithromycin and 250 μM Vitamin C) for 3 days was assessed using the Seahorse XFe96 analyzer. Note that the DAV triple combination inhibits oxidative mitochondrial metabolism (measured by OCR) and induces glycolytic function (measured by ECAR).