Research Paper Volume 11, Issue 7 pp 2138—2150

MiR-204 reduces cisplatin resistance in non-small cell lung cancer through suppression of the caveolin-1/AKT/Bad pathway

Figure 6. miR-204/CAV-1 axis regulates the mitochondrial apoptosis pathway in cisplatin-resistant NSCLC cells. (A) After mitochondria removal, the protein level of cytochrome c in cytosol was measured using western blot analysis. (B) Cleavage of caspase-9 and caspase-3 in CR-A549 and CR-PC9 cells was detected using western blot analysis. (C) Effect of miR-204 (50 pmol/ml), cisplatin (8 μM), and the CVA-1 plasmid (2 μg/ml) on the apoptotic rate of CR-A549 and CR-PC9 cells. *P<0.05 vs. Cisplatin+NCO group. #P<0.05 vs. Cisplatin+miR-204 group.