Research Paper Volume 11, Issue 7 pp 2151—2176

Pyridoxine 5′-phosphate oxidase is correlated with human breast invasive ductal carcinoma development

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Figure 5. The effect of PNPO on breast cell proliferation. (A) The efficiency of lentiviruses infection with GFP and PNPO-shRNA in breast non-cancerous cells (MCF-12A) and cancerous cells (MCF-7 and MDA-MB-231) detected by immunofluorescence microscopy. Scale bar, 500 μm. (B) Effect of PNPO knockdown by PNPO-shRNA on PNPO mRNA expression in MCF-12A, MCF-7, and MDA-MB-231 cells detected by qRT-PCR. (C) Effect of PNPO knockdown by PNPO-shRNA on PNPO protein expression in MCF-12A, MCF-7, and MDA-MB-231 cells detected by Western blot. (D) Histograms show the semi-quantitative analyses of the gels from (C) after densitometry. (E) Effect of PNPO knockdown by PNPO-shRNA on cell viability in MCF-12A, MCF-7, and MDA-MB-231 cells detected by the CCK-8 assay. (F) Effect of PNPO knockdown by PNPO-shRNA on cell cycle in MCF-12A, MCF-7, and MDA-MB-231 cells detected by flow cytometry. (G) Histograms show the semi-quantitative analyses of the cell population in cell cycle phases. Blank, non-infected cells; NC, negative control of shRNA; shPNPO, PNPO-shRNA. n = 3; * P < 0.05.