Research Paper Volume 11, Issue 7 pp 2151—2176

Pyridoxine 5′-phosphate oxidase is correlated with human breast invasive ductal carcinoma development

Figure 5. The effect of PNPO on breast cell proliferation. (A) The efficiency of lentiviruses infection with GFP and PNPO-shRNA in breast non-cancerous cells (MCF-12A) and cancerous cells (MCF-7 and MDA-MB-231) detected by immunofluorescence microscopy. Scale bar, 500 μm. (B) Effect of PNPO knockdown by PNPO-shRNA on PNPO mRNA expression in MCF-12A, MCF-7, and MDA-MB-231 cells detected by qRT-PCR. (C) Effect of PNPO knockdown by PNPO-shRNA on PNPO protein expression in MCF-12A, MCF-7, and MDA-MB-231 cells detected by Western blot. (D) Histograms show the semi-quantitative analyses of the gels from (C) after densitometry. (E) Effect of PNPO knockdown by PNPO-shRNA on cell viability in MCF-12A, MCF-7, and MDA-MB-231 cells detected by the CCK-8 assay. (F) Effect of PNPO knockdown by PNPO-shRNA on cell cycle in MCF-12A, MCF-7, and MDA-MB-231 cells detected by flow cytometry. (G) Histograms show the semi-quantitative analyses of the cell population in cell cycle phases. Blank, non-infected cells; NC, negative control of shRNA; shPNPO, PNPO-shRNA. n = 3; * P < 0.05.