Figure 2. Effects of AAI exposure on the spindle/chromosome structure in porcine oocytes. (A) Representative images of spindle morphologies and chromosome alignment in control and AAI-exposed oocytes. Oocytes were immunostained with anti–α-tubulin-FITC antibody to visualize the spindles and were counterstained with propidium iodide (PI) to visualize the chromosomes. Scale bar, 10 μm. (B) The rate of aberrant spindles was recorded in control and AAI-exposed oocytes. (C) The rate of misaligned chromosomes was recorded in control and AAI-exposed oocytes. Data in (B) and (C) were presented as mean percentage (mean ± SEM) of at least three independent experiments. **P < 0.01.