Research Paper Volume 11, Issue 8 pp 2281—2294

Non-genomic mechanisms mediate androgen-induced PSD95 expression

Figure 4. T-BSA upregulated the phosphorylation level of Erk1/2 and eIF4E through ZIP9/Gnα11. (AC) The ratio of phosphorylated/total levels of P38 (A), JNK (B), and Erk1/2 (C) detected in HT22 cells pre-treated with nc-shRNA or Gnα11-shRNA using Flowmetric Luminex xMAP® assay (n=5). (DF) Western blotting for the phosphorylated/total levels of Erk1/2 and eIF4E induced by T-BSA pre-treated with nc-shRNA, ZIP9-shRNA, or Gnα11-shRNA (n=5). (GJ) Immunofluorescence staining for the phosphorylation level of Erk1/2 and eIF4E induced by T-BSA pre-treated with nc-shRNA, ZIP9-shRNA or Gnα11-shRNA (n=4, scale bars = 20 μm). (n.s.: non-significant; * P < 0.05; ** P < 0.01).