Figure 5. Erk1/2 activation is required in T-BSA-induced eIF4E phosphorylation and PSD95 expression. (A–D) Western blotting for the phosphorylated /total levels of Erk1/2 and eIF4E and the level of synaptic protein PSD95 induced by T-BSA in HT22 cells pre-treated with the Erk1/2 inhibitor SCH772984 (S, 100 nM) for 2 h (n=5). (E–H) Immunofluorescence staining for p-eIF4E and PSD95 induced by T-BSA pre-treated with S (n=4, scale bar = 20 μm). (n.s.: non-significant; * P < 0.05; ** P < 0.01).