Research Paper Volume 11, Issue 8 pp 2327—2342

Cooperative impact of thiazolidinedione and fatty acid synthase on human osteogenesis

Figure 3. Effect of MCC-555 on the Wnt/β-catenin signaling of C3H10T1/2 cells. (A) Western blot analyses. Confluent C3H10T1/2 cells were induced to undergo osteoblastic differentiation for 2, 4, 6, and 8 days with either vehicle (DMSO) or MCC-555 co-treatment. The signals of Ser9-phosphorylated GSK3β were quantitated and normalized to total GSK3β. All the normalized signals were compared to that of the untreated control (U) (to which a value of 1 was assigned). Data represent the mean ± S.D. from three experiments. One-way ANOVA plus Scheffe’s post hoc tests were used to analyze the differences. *, P<0.05 versus the DMSO-treated cells of day 2. Student’s t-test was used to analyze the difference between the DMSO-treated and M5-treated cells of each time point. (B) Western blot analyses. Confluent C3H10T1/2 cells (U) were induced to undergo osteoblastic differentiation for 6h and 24 h at the presence of either DMSO or MCC-555 (5 μM). The signals of the Ser33/37/Thr41-phosphorylated β-catenin were quantitated and normalized to total β-catenin. All the normalized signals were compared to that of the untreated control (to which a value of 1 was assigned). Data represent the mean ± S.D. from three experiments. One-way ANOVA plus Scheffe’s post hoc tests were used to analyze the differences. *, P<0.05 versus the untreated cells.