Figure 7. Size distribution and biomineralization of bmMSC cell clusters. (A) The sizes of cell clusters in the scaffolds incubated in the bioreactor for 7, 14, and 21 days were measured by SEM. One-way ANOVA plus Scheffe’s post hoc tests were used to analyze the differences. *, P<0.05 versus shEV cells of day 7. Student’s t-test was used to analyze the difference between the shFASN and shFASN plus M5-treated cells of days 7, 14 and 21. (B) The cell clusters of shEV, shFASN, and shFASN+M5 groups were categorized by the size (5~20 μm, 21~50 μm, and 51~100 μm), and the percentage was calculated. (C) The cell clusters were stained with xylenol orange to indicate the biomineralized areas, and with Hoechst 33342 to indicate the location of nucleus.