Research Paper Volume 11, Issue 8 pp 2343—2351

Epigenetic control of Foxp3 in intratumoral T-cells regulates growth of hepatocellular carcinoma

Figure 3. Specific target and alteration of DNMT1 levels in T-cells. (A) We used an AAV carrying either DNMT1, or scrambled (as a control) or shDNMT1 under a CD4 promoter (AAV-pCD4-DNMT1, AAV-pCD4-scrambled, AAV-pCD4-shDNMT1) to successfully target T-cells and alter the levels of DNMT1. (B) Direct fluorescence for GFP in transduced cells. (C, D) RT-qPCR (C) and Western blot (D) for DNMT1. *p<0.05. N=5. Scale bars are 20 μm.