Figure 4. CA-30 corrected aberrant lymphocyte subsets in SAMP8 mice. Representative flow cytometric plots for CD4+CD25+Foxp3+ T cells and CD19+ B cells (A). Flow cytometric quantification of CD4+CD25+Foxp3+ T cells (B) and CD19+ B (C) cells in mouse blood. Approximately 5 × 105 whole blood cells for each mouse were harvested, washed, and incubated with antibodies, then quantified via flow cytometry. *P<0.05, **P<0.01, versus the SAMR1 mice, #P<0.05, ##P<0.01, versus the SAMP8 mice by unpaired Student's t-tests and two-way repeated-measures analyses of variance with Tukey multiple comparisons tests. All values are means ± S.D. n=9-11.