Figure 6. Effects of CA-30 on the abundance and diversity of gut microbiota. The relative abundance of the top 20 dominant gut bacterial genus in each group (A). The total abundance of other genera was less than 1%. ▲,▼, and ※ indicate a significant increase or decrease, respectively, in SAMP8 mice relative to SAMR1 mice, and SAMP8 mice administrated with CA-30 relative to SAMP8 mice. The enriched taxa in the gut microbiota of mice are represented in cladograms (B). The central point represents the root of the tree (bacteria), and each ring represents the next lower taxonomic level (phylum to genus). The diameter of each circle represents the relative abundance of the taxon. The most differentially abundant taxa in each group identified by linear discriminant analysis (LDA) scores generated from the Linear discriminant analysis effect size (LEfSe) analysis (C). Weighted principal component analyses (PCA) at the Genus level in each group (D) and average PCA scores (E). *P<0.05, **P<0.01, versus the SAMR1 mice, ###P<0.001, versus the SAMP8 mice by unpaired Student's t-tests. All values are means ± S.D. n=5-6.