Figure 4. Nampt overexpression alleviates MSC senescence. (A) Nampt mRNA expression determined by RT-qPCR in senescent LP MSCs (P10) after Nampt overexpression. (B) Nampt protein expression examined by western blotting. (C) Cellular morphology (scale bar = 20 μm) and quantification. (D) Logarithmic proliferation in Nampt-overexpressing MSCs. (E) Cell population doubling time (PDT). (F) Detection and analysis of cell cycle. (G) Observation of osteogenesis (scale bar = 50 μm) and quantification. (H) Observation of adipogenesis (scale bar = 20 μm) and quantification. (I) SA-β-gal staining (scale bar = 50 μm) and quantification. (J) Gene expression of the senescence-related factor p16INK4a; n = 3 independent experiments. *P < 0.05, **P < 0.01.