Research Paper Volume 11, Issue 11 pp 3505—3522

Nicotinamide phosphoribosyltransferase postpones rat bone marrow mesenchymal stem cell senescence by mediating NAD+–Sirt1 signaling

Figure 5. Nampt regulation on MSC senescence is linked to the NAD+–Sirt1 signaling pathway. (A, B, C) Intracellular NAD+ content (A) NAD+/ NADH ratio (B) and Sirt1 deacetylase activity (C) were measured when Nampt was suppressed by either FK866 treatment or gene silencing. (D, E, F) Effect of Nampt overexpression on intracellular NAD+ content (D) and NAD+/ NADH ratio (E), as well as Sirt1 deacetylase activity (F); n = 3 independent experiments. *P < 0.05, **P < 0.01.