Figure 1. TRIM6 expression is induced in the heart after MI/R injury. (A–D) Mice were subjected to sham surgery or experimental MI/R. Each group contained 7 mice. At different time periods after reperfusion, the hearts were harvested for analyses. (A) The mRNA level of Trim6 in the heart was determined by qRT-PCR analysis. Actb was used as an endogenous control. Results relative to sham are shown. (B) The protein level of TRIM6 in the heart was determined by Western blotting analysis. β-Actin was used a loading control. The representative images (left) and band intensity relative to sham (right) are shown. (C) The expression of TRIM6 in the heart slices at 24 hrs after reperfusion was detected by immunohistochemistry (IHC). Scale bar, 100 μM. (D) The quantification of TRIM6 staining shown in (C) was shown as percentage of positive staining cardiomyocytes in the heart slices. Data are expressed as mean ± SD (n = 7). **, P < 0.01; *, P < 0.05 vs. sham group.