Research Paper Volume 11, Issue 11 pp 3574—3584

TGF-β mediates aortic smooth muscle cell senescence in Marfan syndrome

Figure 1. VSMCs exhibit senescence in aortic aneurysm tissue from MFS patients. (A) Representative images of HE stained sections of ascending aorta from control donors and aortic aneurysm from MFS patients. Note the degeneration of the medial layer of the aortic wall in MFS patients. The box shows the location of the magnified region. Scale bar=200 μm. (B) Western blot and quantitative analysis of p53 and p21 levels in the ascending aorta of control donors and MFS patients. (C) Representative images and quantitative analysis of SA-β-gal staining in the ascending aorta from control donors and MFS patients. The box shows the location of the magnified region. Scale bar=200 μm. (D) Representative images and quantitative analysis of p53 (red) and α-SMA (green) staining in the ascending aorta from control donors and MFS patients. Scale bar=50 μm. Data are expressed as the mean±SEM; n=6. ***p<0.001.