Research Paper Volume 11, Issue 11 pp 3574—3584

TGF-β mediates aortic smooth muscle cell senescence in Marfan syndrome

Figure 2. VSMCs isolated from the ascending aorta of MFS patients exhibit cellular senescence. (A) Representative images of immunofluorescent staining for α-SMA and calponin in control- and MFS-VSMCs. Scale bar=50 μm. (B) Representative light micrographs of control- and MFS-VSMCs. Cell size is expressed relative to control. Scale bar=100 μm. (C) Representative images and quantitative analysis of SA-β-gal staining in control- and MFS-VSMCs. Numbers of SA-β-gal-positive cells are expressed as percentages of the total numbers of control- or MFS-VSMCs. Scale bar=100 μm. (D) Representative images and quantitative analysis of immunofluorescent ki-67 staining in control- and MFS-VSMCs. Numbers of ki-67-positive cells are expressed as percentages of the total numbers of control- or MFS-VSMCs. Scale bar=100 μm. (E) Western blotting and quantitative analysis of p53 and p21 levels in control- and MFS-VSMCs. (F) Concentrations of IL-6, IL-8, TNF-α and INF-γ in medium conditioned by control- or MFS-VSMCs. Data are expressed as the mean±SEM. n=3. ***p<0.001.