**Figure 4.** **TGF-β1 induces cellular senescence in VSMCs through elevation of ROS.** (**A**) Representative images and quantitative analysis of DHE staining in the ascending aorta of control donors and MFS patients. ROS levels were analyzed and expressed relative to control. Scale bar=200 μm. n=6. (**B**) Representative images and quantitative analysis of Mito-sox staining of control- and MFS-VSMCs. ROS levels were analyzed and expressed relative to control. n=3. Scale bar=100 μm. (**C**) Representative images and quantitative analysis of Mito-sox staining in control-VSMCs left untreated or treated with TGF-β1 or TGF-β1 combined with Mito-tempo. ROS levels was analyzed and expressed relative to control. n=3. Scale bar=100 μm. (**D**) Representative images and quantitative analysis of SA-β-gal staining in control-VSMCs left untreated or treated with TGF-β1 or TGF-β1 combined with Mito-tempo. n=3. Scale bar=100 μm. Numbers of SA-β-gal-positive cells are expressed as percentages of the total cells. Data are expressed as the mean±SEM. ****p<0.001.*