Research Paper Volume 11, Issue 11 pp 3574—3584

TGF-β mediates aortic smooth muscle cell senescence in Marfan syndrome

Figure 4. TGF-β1 induces cellular senescence in VSMCs through elevation of ROS. (A) Representative images and quantitative analysis of DHE staining in the ascending aorta of control donors and MFS patients. ROS levels were analyzed and expressed relative to control. Scale bar=200 μm. n=6. (B) Representative images and quantitative analysis of Mito-sox staining of control- and MFS-VSMCs. ROS levels were analyzed and expressed relative to control. n=3. Scale bar=100 μm. (C) Representative images and quantitative analysis of Mito-sox staining in control-VSMCs left untreated or treated with TGF-β1 or TGF-β1 combined with Mito-tempo. ROS levels was analyzed and expressed relative to control. n=3. Scale bar=100 μm. (D) Representative images and quantitative analysis of SA-β-gal staining in control-VSMCs left untreated or treated with TGF-β1 or TGF-β1 combined with Mito-tempo. n=3. Scale bar=100 μm. Numbers of SA-β-gal-positive cells are expressed as percentages of the total cells. Data are expressed as the mean±SEM. ***p<0.001.