Figure 1. MORC2 is highly expressed in CCA and is associated with poor prognosis. (A) The expression of MORC2 mRNA was determined in 44 pairs of CCA samples. (B) The expression of MORC2 in paired CCA samples from cohort 2 was confirmed by immunohistochemical staining. Representative examples of MORC2 staining are shown. (C) The protein expression of MORC2 was examined in 8 pairs of CCA tumor tissues (T) and corresponding normal tissues (N). (D) The relevant density of MORC2 protein was compared, and the results are shown in the scatter plot. (E) Kaplan-Meier survival analyses were conducted to evaluate the influence of MORC2 on overall survival. (F–G) mRNA and protein expression levels of MORC2 were evaluated by RT-qPCR and western blotting in six CCA cell lines. ***P < 0.001, **P < 0.01, *P < 0.05.