Figure 2. Knockdown of MORC2 suppresses CCA cells proliferation in vitro. (A) MORC2 protein levels in stable HuCCT1 and RBE cells. Each cell line was divided into the following three groups: the sh-NC group (cells infected with Lv-sh-CTRL), the sh-MORC2-1 group (cells infected with Lv-sh-MORC2-1) and the sh-MORC2-2 group (cells infected with Lv-sh-MORC2-2). (B–C) Cell Counting Kit-8 assay of cell proliferation in MORC2 stable knockdown HuCCT1 and RBE cells; cell viability was determined at 24, 48, 72 and 96 h. (D) Representative images and quantitative clonogenic analysis of plate colony formation in CCA cells. (E–F) DNA synthesis was analyzed in CCA cells transfected with the corresponding shRNA via an EdU incorporation assay. (G) The expression levels of p-AKT and AKT in MORC2-knockdown HuCCT1 and RBE cells were examined by western blotting. All experiments were performed in triplicate, **P < 0.01, *P < 0.05.