Figure 5. MORC2 is a direct target of miR-186-5p in CCA cells. (A) Analysis by TargetScan and miRanda target prediction algorithms revealed that the 3' UTR of MORC2 contains putative binding sites for multiple miRNAs. (B–C) qRT-PCR analysis of the expression levels of MORC2 after transfection with a miR-186-5p mimic or its inhibitor in HuCCT1 and RBE cell lines. (D) Relative expression levels of miR-186-5p in adjacent normal tissues and CCA tissues. (E) Correlation between miR-186-5p expression and MORC2 expression in clinical samples. (F) Expression levels of miR-186-5p in indicated CCA cell lines. (G) Schematic representation of potential binding sites of miR-186-5p with WT or Mut MORC2. (H–I) Luciferase activity of WT or Mut MORC2 after cotransfection of a luciferase construct fused with the wild-type or site mutant 3′-UTR of MORC2 and pre-miR-186-5p or miR-NC. All experiments were performed in triplicate, ***P < 0.001, **P < 0.01, *P < 0.05.