Research Paper Volume 11, Issue 11 pp 3639—3649

MORC2 promotes cell growth and metastasis in human cholangiocarcinoma and is negatively regulated by miR-186-5p

Figure 6. miR-186-5p is involved in MORC2-mediated CCA cell growth and metastasis. (A) CCK-8 analysis of cell viability after transfection with miR-186-5p inhibitor and sh-MORC2 plasmid in the HuCCT1 cell line. (BC) Transwell analysis of cell invasive potential after transfection with miR-186-5p inhibitor and sh-MORC2 plasmid in the HuCCT1 cell line. (DE) Western blot analysis of the levels of p-AKT, AKT, Slug, E-cadherin, N-cadherin and Vimentin after transfection with miR-186-5p inhibitor and sh-MORC2 plasmid in the HuCCT1 cell line. All experiments were performed in triplicate, **P < 0.01, *P < 0.05.