Research Paper Volume 11, Issue 11 pp 3731—3749

Nectandrin B-mediated activation of the AMPK pathway prevents cellular senescence in human diploid fibroblasts by reducing intracellular ROS levels

Figure 3. Flow cytometry for analysis of cell cycle and apoptosis. Graph shows the relative percentage of cells of the indicated genotype in the subG1, G0/G1, S, and G2/M phases of the cell cycle. Young and old HDFs were treated with either vehicle or NecB (10 or 20 μg/mL) for 16 h, stained with PI, and then analyzed by flow cytometry.