Research Paper Volume 11, Issue 11 pp 3811—3823

Figure 2. Silencing of AGAP2-AS1 represses proliferation and invasion, and promoted apoptosis in GBM cells. U87/MG and U251/MG cells were transfected with si-NC or si-AGAP2-AS1, while A172 cells were introduced with empty vector or pcDNA-AGAP2-AS1. (A) qRT-PCR analysis of transfection efficiency in U87/MG, U251/MG and A172 cells. (B) CCK-8 analysis was conducted to detect the viability in transfected U87/MG, U251/MG and A172 cells. (C–E) (d) EdU staining assay was performed to evaluate the proliferation ability in transfected U87/MG, U251/MG and A172 cells. Red, EdU staining for dividing cell; blue, DAPI staining for nuclear. (F–H) Colony-forming assay was used to examine the cloning ability in transfected U87/MG, U251/MG and A172 cells. (I–K) Transwell assay was carried out to assess the invasiveness in transfected U87/MG, U251/MG and A172 cells. (L and M) Flow cytometry analysis was applied to determine the apoptotic rate in transfected U87/MG, U251/MG and A172 cells. *P < 0.05, **P < 0.01.