Research Paper Volume 11, Issue 11 pp 3811—3823

Long non-coding RNA AGAP2-AS1 exerts oncogenic properties in glioblastoma by epigenetically silencing TFPI2 through EZH2 and LSD1

Figure 5. AGAP2-AS1 promotes GBM progression partially through epigenetically inhibiting TFPI2 expression in vitro. U87/MG cells were transfected with si-NC, si-AGAP2-AS1, or co-transfected with si-AGAP2-AS1 and si-TFPI2. (A) Western blot analysis of TFPI2 protein level in transfected cells. (B) CCK-8 assay of proliferation ability in transfected cells. (C) Colony formation assay of cloning ability in transfected cells. (D) Transwell assay of invasiveness in transfected cells. (E) Flow cytometry analysis of apoptosis in transfected cells. *P < 0.05, **P < 0.01.