Figure 5.AGAP2-AS1 promotes GBM progression partially through epigenetically inhibiting TFPI2 expression in vitro. U87/MG cells were transfected with si-NC, si-AGAP2-AS1, or co-transfected with si-AGAP2-AS1 and si-TFPI2. (A) Western blot analysis of TFPI2 protein level in transfected cells. (B) CCK-8 assay of proliferation ability in transfected cells. (C) Colony formation assay of cloning ability in transfected cells. (D) Transwell assay of invasiveness in transfected cells. (E) Flow cytometry analysis of apoptosis in transfected cells. *P < 0.05, **P < 0.01.
