Research Paper Volume 11, Issue 11 pp 3851—3863

Time-restricted feeding delays the emergence of the age-associated, neoplastic-prone tissue landscape

Figure 2. The growth of transplanted pre-neoplastic hepatocytes in the liver of rats exposed to ALF or TRF regimens for 18 months. Following transplantations, animals from both groups were continued on ALF for 3 months (see Methods for details). Larger class clusters of DPP-IV expressing, pre-neoplastic hepatocytes (A, B and C) were significantly more frequent in ALF compared to TRF group (*p <0.05 and **p <0.01). (D and E) H&E staining of livers from ALF or TRF groups, respectively. Note the presence of cholangio-fibrosis, more prominent in ALF group. Magnification: A and B, 200x; D and E, 100x.