Figure 4. The effect of TRF on markers of systemic aging in rats exposed to ALF or TRF for 18 months and continued on ALF for additional 3 months. (A) Plasma levels of IGF-1 were decreased in TRF group (p-value <0.05). (B and C) BDNF levels in the hippocampus and plasma; differences were statistically significant in the hippocampus (*p-value <0.05). (D) Plasma triglycerides, LDL and HDL levels in rats after 1 or 2 years on ALF regimen. A significant increase in all parameters was seen at 2 years (**p <0.01; ***p <0.001). (E) Plasma lipids profile in rats exposed to ALF or TRF for 18 months and continued on ALF for additional 3 months. Rats receiving TRF had lower levels of triglycerides, LDL and HDL (*p-value <0.05; **p-value <0.01).