Research Paper Volume 11, Issue 13 pp 4338—4353

Targeting HMGB1 by ethyl pyruvate ameliorates systemic lupus erythematosus and reverses the senescent phenotype of bone marrow-mesenchymal stem cells

Figure 5. (A) 30 MRL/lpr mice were divided into three groups: MRL/lpr mice treated with normal saline, Ep-40mg/kg-treated MRL/lpr mice and Ep-100mg/kg -treated MRL/lpr mice. Ep was injected intraperitoneally to treat MRL/lpr mice aged 14 weeks for 8 weeks. (B) The expressions of HMGB1 in serum of MRL/lpr mice were examined by Elisa. (C) Survival curves observed that the survival rate of Ep-40mg/kg group and Ep-100mg/kg group was higher than that of control group. (D) Three groups MRL/lpr mice were weighed one time two weeks. (E) 24-hours urinary protein was measured by coomassie brilliant blue method. (F) HE-staining showed that renal pathological changes of MRL/lpr mice were significant, including glomerular sclerosis, mesangial cell proliferation, matrix widened, and lymphocytes infiltrating the interstitium. However, histopathological changes of other groups were alleviated. (Bar represents mean ± SD,*P < 0.05 compared with the control group, #P < 0.05 compared with the control group) (Con=Control group).