Research Paper Volume 11, Issue 13 pp 4338—4353

Targeting HMGB1 by ethyl pyruvate ameliorates systemic lupus erythematosus and reverses the senescent phenotype of bone marrow-mesenchymal stem cells

Figure 6. (A, B) the size comparison of spleen in three groups: MRL/lpr mice treated with normal saline, Ep-40mg/kg-treated MRL/lpr mice and Ep-100mg/kg -treated MRL/lpr mice. (C, D) Treg cell analysis in cells obtained from lymph nodes, spleen and peripheral blood. EP treatment upregulated the number of Treg cells. (E, F) BM-MSCs from MRL/lpr mice were isolated, then were fixed and stained for SA-β-gal. (G, H) Cell viability was assessed by flow cytometry analysis. (I, J) Expressions of TLR4, p-p65, and IκBa in MSCs from MRL/lpr mice, normal group and EP-treated group by Western blot analysis. (Bar represents mean ± SD,*P < 0.05 compared with the control group) (Con=Control group).