Research Paper Volume 11, Issue 13 pp 4354—4366

Ammonia induces calpain-dependent cleavage of CRMP-2 during neurite degeneration in primary cultured neurons

Figure 3. CRMP-2 is cleaved after ammonia treatment, which is mediated by calpain. Cultured cerebellar granule cells were treated as in Figure 1, and then neuronal lysates were subjected to western blotting with antibodies against CRMP-2 and αII-Spectrin (A). (B) Quantification of intensity bands for cleaved CRMP-2 relative to that for tubulin. Relative value in control group was set to 100%. Western blotting analyses of cultured cerebellar granule cells treated along with calpain inhibitor ALLM (10 μM) (C and D), caspase inhibitor ZVAD (20 μM) (E and F), and GSK-3 inhibitor AR-A014418 (10 μM) (G and H). Data are means ± SEs from at least three independent experiments; * p < 0.05 vs. Ctrl.