Research Paper Volume 11, Issue 13 pp 4354—4366

Ammonia induces calpain-dependent cleavage of CRMP-2 during neurite degeneration in primary cultured neurons

Figure 5. Inhibition of calpain prevents ammonia-induced impairment of synaptic activity. Cultured cerebellar granule cells were treated with ammonia (0, 2, 5, or 10 mM) for 24 h and subjected to patch-clamp electrophysiological recording. Representative tracings (A) and quantification of frequencies and amplitudes (B) of mEPSCs. Representative tracings (C) and quantification of frequencies and amplitudes (D) of mEPSCs in neurons treated with 2 mM ammonia for 24 h with or without ALLM, ZVAD, or AR-A014418. Data are means ± SEs; n =15 cells from three cultures from seven animals; * p < 0.05 vs. Ctrl.