Research Paper Volume 11, Issue 13 pp 4407—4437

HNRNPA1-mediated 3′ UTR length changes of HN1 contributes to cancer- and senescence-associated phenotypes

Figure 6. Knockdown of HN1 and HNRNPA1 inhibits ability of colony formation and cell migration. (A) The colony formation capacity between control (Ctrl) and HN1-KD (two shRNAs) A549 cells was detected by crystal violet staining. (B) Cell migration ability in HN1-KD (two shRNAs) A549 cells was detected by transwell assay. (CD) The ability of colony formation (C) and cell migration (D) was weakened in HNRNPA1-KD (two shRNAs) A549 cells. (EF) Cell migration ability was weakened in HNRNPA1-KD (E, two shRNAs) and HN1-KD HUVECs (F, two shRNAs) when comparing to control cells, as evaluated by transwell migration assay.