This paper was originally published in Aging Advance Online Publications on July 4, 2019.
In compliance with Aging's withdrawal policy, the paper was withdrawn in its entirety.
Figure 4. MiRNA let-7a respectively binds to LINC00265 and IL-6 genes. (A) The identification of VMSC by α-smooth muscle actin. (B) The position of LINC00265 in VSMCs. (C) Construction of normal and mutant sequences of IL-6 gene and LINC00265. (B–D). Luciferase activity reflecting IL-6 transcriptional activity. Mimic NC+IL-6-WT: invalid let-7a overexpression+lL wild type sequence; Mimic-NC+IL-6 Mut: pseudo let-7a overexpression+IL-6 mutant sequence; let-7a mimic+IL-6-WT: let-7a overexpression+IL-6 wild type sequence; Mimic NC+lL-6-Mut: let-7a overexpression+lL mutant sequence; Mimic NC: invalid let-7a overexpression; Mimics let-7a: let-7a overexpression; LINC00265 wt: LINC00265 wild type sequence; LINC00265-mut: LINC00265 mutant sequences; IL-6-WT: IL-6 wild type sequence; IL-6-Mut: IL-6 mutant gene sequence; pcDNA3.1-LINC00265: the overexpression sequence of LINC00265; pcDNA3.1-NC: meaningless LINC00265 antisense carrier. VSMCs: vascular smooth muscle cells. *P < 0.05, **P < 0.01.