Figure 5. Effects of overexpression or knockout of LINC00265 and let-7a analogues on IL-6 content in cultured smooth muscle cells in vitro. (A) Effects of overexpression of LINC00265 and let-7a analogues on IL-6 content in cultured smooth muscle cells in vitro, immunofluorescence of lL-6 in vascular smooth muscle cells of abdominal aorta (green fluorescence represented cytoplasm, blue fluorescence represented nucleus, Red fluorescence represents intracellular IL-6 protein). (B) Quantitative analysis of IL-6 in overexpression of LINC00265 and let-7a analogues in vascular smooth muscle cells of aorta. (C) Effects of knockout of LINC00265 and let-7a analogues on IL-6 content in cultured smooth muscle cells in vitro, immunofluorescence of lL-6 in vascular smooth muscle cells of abdominal aorta (green fluorescence represented cytoplasm, blue fluorescence represented nucleus, Red fluorescence represents intracellular IL-6 protein). (D) Quantitative analysis of IL-6 in knockout of LINC00265 and let-7a analogues in vascular smooth muscle cells of aorta. *P < 0.05, **P < 0.01.