Research Paper Volume 11, Issue 13 pp 4597—4610

Role of microtubule-associated protein 6 glycosylated with Gal-(β-1,3)-GalNAc in Parkinson's disease

Figure 6. Confirmation glycoprotein of interest is MAP6. (A) Western blot analysis of soluble lysates and insoluble precipitates. M, protein size standards. (B) Densitometric analysis of MAP6 levels normalized to the level of β-actin in soluble lysates and insoluble precipitates. Bars depict the mean±SD; * p<0.05 vs. control. (C) Blots showing that MAP6 is a glycoprotein containing Gal-(β-1,3)-GalNAc oligosacchrides. Immunoprecipitation was coupled with Western blot analysis of extract from the whole mouse brain. The SDS-PAGE gel was visualized using silver-staining kit; and the membrane of WB was blotted with ABL, and visualized using a ECL kit.